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. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jul 25;388(1):56–61. doi: 10.1016/j.bbrc.2009.07.112

Figure 1.

Figure 1

ZFN-mediated gene targeting in HEK293 cells. (A) Schematic representation of ZFN-mediated gene correction of the mutant eGFP locus (disrupted with either the hCCR5 or mTYR ZFN target sites) in HEK293 Flp-In cells. ZFN-mediated gene correction of the mutant eGFP locus using hCCR5-OHD3-FN, 3-finger hCCR5-specific ZFNs containing the FokI nuclease domain variants reported by Miller et al. (19) (B) and mTYR-OHD1-FN, 3-finger mTYR-specific ZFNs containing the FokI nuclease domain variants generated at PBPL (C). GFP positive cells were seen 5 days post-transfection of HEK293 cells with 3-finger ZFNs and donor plasmid. No GFP positive cells were seen by microscopy after transfection of HEK293 cells with donor plasmid alone. OHD depicts obligate heterodimers.