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. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jul 25;388(1):56–61. doi: 10.1016/j.bbrc.2009.07.112

Figure 3.

Figure 3

Phenotypic and genotypic changes in the albino mouse melanocytes. A) Four different tyrosinase gene-corrected pigmented melanocytes 6 days post nucleofection of Melan-C cells (albino melanocytes) with the TYR ZFNs and the correcting plasmid (Tyrs J), are shown. B) Three pigmented clones (that had originated from individual pigmented cells) were obtained and correction of the mTYR gene was monitored by RFLP analysis (18). Presence of both 179 bp (a marker for the wild-type) and 144 bp (a marker for the mutant allele) fragments suggest targeted correction of one of the mutant tyrosinase alleles in the pigmented mouse albino melanocytes.