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. Author manuscript; available in PMC: 2010 Oct 9.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jul 25;388(1):56–61. doi: 10.1016/j.bbrc.2009.07.112

Table 1. Genotypic analysis of the eGFP, CCR5 and CCR2 loci in 4 different GFP+ clones.


Mutant eGFP locus 		        BstXI        CCR5 sequence            BstXI
...CAGCCGCTACCCATAATAATGGGTCATCCTCATCCTGATAAACTGCAAAAGCCATGATGATGGAAGCAGCACGA...
Transduced eGFP locus .....................CAGCCGCTACCCCGACCACATGAAGCAGCACGA.....................WT
.....................CAGCCGCTACCCCGACCACATGAAGCAGCACGA.....................GFP+1 (5)
.....................CAGCCGCTACCCCGACCACATGAAGCAGCACGA.....................GFP+2 (5)
.....................CAGCCGCTACCCCGACCACATGAAGCAGCACGA.....................GFP+3 (5)
.....................CAGCCGCTACCCCGACCACATGAAGCAGCACGA.....................GFP+4 (6) *
#Endogenous CCR5 locus           ZFN-L      ZFN-R
..................GGTCATCCTCATCCTGATAAACTGCAAAAGG.................................WT
..................GGTCATCCTCATCCTGATctgatAAACTGCAAAAGG.................................GFP+1 (5)
..................GGTCATCCTCATCCTGATctgatAAACTGCAAAAGG.................................GFP+2 (5)
..................GGTCATCCTCATCCTGATctgatAAACTGCAAAAGG.................................GFP+3 (5)
..................GGTCATCCTCAT---------------AACTGCAAAAGG.................................GFP+4 (4) *
..................GGTCATCCTCATCC------------AACTGCAAAAGG.................................GFP+4 (2) *
Endogenous CCR2 locus         ZFN-L          ZFN-R
..................TGGTCGTCCTCATCTTAATAAACTGCAAAAAGC...........................WT
..................TGGTCGTCCTCATCTTAATAAACTGCAAAAAGC...........................GFP+1 (5)
..................TGGTCGTCCTCATCTTAATAAACTGCAAAAAGC...........................GFP+2 (5)
..................TGGTCGTCCTCATCTTAATAAACTGCAAAAAGC...........................GFP+3 (5)
..................TGGTCGTCCTCATCTTAATAAACTGCAAAAAGC...........................GFP+4 (6) *
*

The number of recombinant E. coli clones containing the PCR fragment from each of the GFP+ clone that were sequenced are shown in brackets. Sequences of six recombinant E. coli clones were determined for the GFP+4 clone and 5 for the rest.

#

NHEJ mutants with bp deletions at the CCR5 locus are depicted by dashes (-), While mutants with bp insertions are shown by bold type and lower case letters.