TABLE 1.
PCR primers used for cloning of Rv1636, Rv0138, Rv2524, esxI, Rv3740, and hspX
| Locus tag or gene | Primer sequence (5′-3′) | Restriction Site |
|---|---|---|
| Rv1636 | up-GGATCCATGAGCGCCTATAAGACCGTGG | BamHI |
| low-GAATTCCTAGGTGGTGTGCACGATCAGCA | EcoRI | |
| Rv3740 | up-GGATCCATGTCACCGATCGATGCGCT | BamHI |
| low-AAGCTTTCATAGCCCGACCGCGCGCT | HindIII | |
| esxI | up-GGATCCATGACCATCAACTATCAATT | BamHI |
| low-AAGCTTTTAGGCCCAGCTGGAGCCGA | HindIII | |
| Rv0138 | up-GGATCCGTGAGCGCTTCGGAGTTCTCC | BamHI |
| low-AAGCTTTTAAGGACCTCCATGCCGGCG | HindIII | |
| Rv2524 | up-GGATCCGTCGGCGGCCAGATCCCGAC | BamHI |
| low-AAGCTTTCACAGTTGAGCCTTGCCCAACCGGA | HindIII | |
| hspX | up-GGATCCGGATCCATGGCCACCACCCTTCCCGTTC | BamHI |
| low-AAGCTTAAGCTTTCAGTTGGTGGACCGGATCTGA | HindIII |