FIG. 1.
Immunochromatographic test strip. (A) Schematic representation of the strip. The nitrocellulose membrane strip consists of anti-mannan IgG and anti-rabbit IgG antibodies that are immobilized as a test line and a control line, respectively. The conjugated pad contains the colloidal gold-anti-mannan IgG conjugate. The proximal and distal ends of the nitrocellulose membrane are covered with the sample pad and the absorption pad, respectively. (B) Representative results. Suspensions of C. albicans ATCC 10231 at cell densities of 103 to 107 CFU/ml were heated in antigen extraction buffer and subjected to immunochromatography for 20 min. The sample with double red lines is Candida positive, and that with a single red line only at the control line is negative. Data for other Candida species are not shown. Only a portion of the strip is shown.