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Correlation between the amount of Candida mannan and the intensity of the test line. Purified C. albicans mannan was serially diluted and then subjected to immunochromatography. The test line was traced by a densitometer at 525 nm. The absorbance was plotted against the amount of mannan quantified by the phenol sulfuric acid method with d(+)-mannose as the reference (3).
