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GK1 enhances the secretion of proinflammatory cytokines resulting from cognate interactions between activated DCs and T cells. Supernatants from the cocultures of LPS-treated DCs or LPS- and GK1-treated DCs obtained from the experiment whose results are shown in Fig. 2B were recovered, and the cytokine levels were determined by flow cytometry with a cytometric bead array mouse inflammation kit (BD Biosciences). The results represent the mean of two independent experiments.
