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. Author manuscript; available in PMC: 2010 Jun 1.
Published in final edited form as: Mol Cancer Res. 2009 May 26;7(6):897–906. doi: 10.1158/1541-7786.MCR-08-0519

Figure 3.

Figure 3

Effect of ED on cellular sensitivity to gemcitabine and troxacitabine. Colony formation efficiency and percent survival was determined as described in Figure 1 and Materials and Methods, following exposure to the indicated doses of gemcitabine (A) or troxacitabine (B). Shown is the average and standard deviation of at least 5 data points from 3 independent experimental runs. (C) DNA strand break levels in ED8 and T-REx cell lines following exposure to troxacitabine. After tet induction, ED8 and T-REx cells were treated with 0, 10 or 30 μg/ml troxacitabine for 24 hr and subsequently processed for Comet analysis (see Materials and Methods). Shown is the average and standard deviation of the OTM determined from a minimum of 50 cells from 3 independent experimental slides. *, P < 0.002; **, P < 0.01. Note: T-Rex cells without tet or troxacitabine treatment were found to have a similar OTM to tet-exposed T-Rex controls (data not shown).