Table 1.
Conservation of phosphorylation motifs in PrP amongst various species
| Putative motif | Phospho AA | Mammals | Chicken | Turtle | Doppel |
|---|---|---|---|---|---|
| PGQSPGGN | S43 | All | RQ | SN | |
| IHFGSDYED | S143 | Only in human, bovine, and bison | S | R | A |
| FGSDYEDRY | Y145 | W in rat, mouse, and hamster | D | E | |
| YEDRYYREN | Y149 | All | WW | WW | YY |
| PMDEYSNQN | Y169 | All | R | A | |
| HTVTTTTKG | T191 | All | P | P | F |
| TVTTTTKGE | T192 | All | A | N | Q |
| VTTTTKGEN | T193 | All | A | E | K |
| YQRGSSMVL | S230 | All except rabbit | S | S | A |
| QRGSSMVLF | S231 | All except rabbit | G | G | G |
| VLFSSPPVI* | S237 | All | AD | DP | HQ |
Mammalian species examined are human, guar, macaque, kudu, bison, rhesus monkey, baboon, mink, rabbit, camel, rat, nilgai, sheep, mouse, hamster, cattle, and cat. Human Doppel was also included. The phosphorylation sites were identified with NetPhos 2.0 Server in EXPASY and had significant scores, except the last SP motif (*), which has low predictability for phosphorylation.