FIGURE 1.

Representative photomicrographs of kidney injury molecule-1 (Kim-1) immunoreactivity in kidneys of rats treated with nephrotoxicants. No Kim-1 expression was found in the cytoplasm of epithelial cells of all segments in the cortex in rats treated with dH₂0 vehicle (A). In the time course study with gentamicin (Gen), Kim-1 expression was detected on the surface of epithelial cells in the S1/S2 segments in rats 24 hours after the 3rd daily injection of 100 mg Gen/kg, sc (B). Kim-1 expression was also seen in the cytoplasm of necrotic and dying epithelial cells in the S1/S2 segments in rats 72 hours after the 3rd daily injection of Gen 100 mg/kg, sc (C). Kim-1 was observed in the cytoplasm of desquamated necrotic cells in the lumen of these segments 24 hours after the 3rd injection of Gen 300 mg/kg (D). In the time course studies with mercury (Hg) or chromium (Cr), Kim-1 expression was detected in rats treated with Hg 0.25 mg/kg, iv, after 24 hours (E) and 72 hours (F) or Cr 5 mg/kg after 24 hours (G) and 72 hours (H). Original magnification of all figures: ×400.