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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: J Neurochem. 2009 May 29;110(3):990–1004. doi: 10.1111/j.1471-4159.2009.06194.x

Fig 2. The effect of calpeptin on DCD in neurons imaged with Fluo-4FF.

Fig 2

Neurons loaded with the membrane potential sensitive dye TMRM+ (red) and the calcium indicator Fluo-4FF (green) are depicted before (a) and 16 minutes after (b) treatment with 100 μM glutamate. Arrows identify examples of cells with intense Fluo-4FF fluorescence that underwent calcium deregulation while the arrowhead denotes a cell that did not yet exhibit DCD although it depolarized in response to glutamate. In (c-d), Fluo-4FF responses in individual cells from representative fields of neurons are plotted over time in the absence (c) and presence (d) of 10 μM calpeptin. (e) shows the average [Ca2+]c response from each field of cells.