Table 1.
Treatment | % O2 of culture | % DCD at 20 min | % DCD at 60 min | # of cells | # of view fields |
---|---|---|---|---|---|
calpeptin (10 μM) | 3 | 109.9 ± 6.7* | 115 ± 6.4* | 705 | 27 |
PD150606 (10 μM) | 3 | 68.2 ± 14.0 | 83.1 ± 8.4 | 384 | 6 |
PD150606 (50 μM) | 3 | 11.8 ± 4.8* | 44.7 ± 11.8* | 300 | 6 |
KB-R7943 (10 μM) | 3 | 31.4 ± 10.7* | 57.6 ± 11.7* | 348 | 6 |
calpeptin (10 μM) | 20 | 125.7 ± 10.6* | 112.2 ± 4.3* | 590 | 18 |
PD150606 (10 μM) | 20 | 71.8 ± 7.1* | 92.0 ± 6.2 | 303 | 6 |
PD150606 (50 μM) | 20 | 17.0 ± 3.4* | 41.0 ± 6.1* | 302 | 5 |
KB-R7943 (10 μM) | 20 | 56.1 ± 9.4* | 77.8 ± 10.9 | 312 | 6 |
Nifedipine (1 μM) was present in experiments with KB-R7943 to exclude the possibility that action was due to effects on voltage-dependent Ca2+ channels. Significance was determined by comparing the relative incidence of DCD in n view fields to 100% using a two-tailed Student's t-test.
p<0.05