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. Author manuscript; available in PMC: 2009 Oct 11.
Published in final edited form as: J Med Chem. 2009 Jun 11;52(11):3539–3547. doi: 10.1021/jm900116d

Figure 8. Compound 11 does not induce apoptosis in organotypic slices.

Figure 8

Slices prepared from JNPL3 transgenic mice were incubated with either DMSO vehicle control or varying concentrations of 11 for seven days, then extracted and analyzed for levels of α-tubulin and cleaved PARP1. (a) Representative immunoblots collected after incubation with DMSO vehicle control (C) or 11 (T) are shown. (b) Levels of cleaved PARP1 (hollow bar) and α-tubulin (solid bar) immunoreactivity were quantified by densitometry (n = three observations) and plotted as a normalized percentage relative to treatment with vehicle alone. Cleaved PARP1 (CP) levels were unaffected after exposure to 11, suggesting that executioner caspase activity was not activated even at the highest concentration tested. Similarly, efficacious concentrations of 11 (1 nM) did not modulate levels of α-tubulin immunoreactivity in tissue extracts. However, high concentrations of 11 associated with induction of tau aggregation (1 μM) led to marked decreases in α-tubulin immunoreactivity. *, p < 0.05; **, p < 0.01 when compared to slices treated with vehicle alone by Student’s t test.