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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Nat Neurosci. 2009 Aug 16;12(9):1129–1135. doi: 10.1038/nn.2372

Figure 3. Post-translational state of 4E-BP activity in parkin/PINK1 mutants.

Figure 3

(a) Western blot analysis of phosphorylated and non-phosphorylated 4E-BP levels in adult tissue from control, parkin, and PINK1 mutant flies. Thor2 mutant flies were used as a negative control. (b) Relative levels of phosphorylated and non-phosphorylated 4E-BP after normalization for total levels of 4E-BP. (c) Western blots for phosphorylated and non-phosphorylated Akt1 in control, parkin and PINK1 mutant adult tissue. (d) Quantified proportion of phospho-Akt1 relative to total Akt1. Charts show mean and SEM of at least three independent experiments. Significance was determined by Student’s t-test with Bonferroni correction (***P<0.001, *P<0.05).