Table 1.

Mass spectral analysis of purified cytosolic rat liver ω-amidase.

Gel Band #	Protein Hit #	Accession #	Protein Name	Mascot Score	Protein Mass	% Coverage	# of Significant Peptides	Expectation Values Range
1a	1	77628000	Nitrilase family, member 2	584	31024	26	7	5.3 × 10−10 to 0.003
2a	1	204499	Glutathione S-transferase Y-b subunit (EC 2.5.1.18)	104	22042	17	2	1.2 × 10−6 to 0.011
	2	33356830	Crystal structures of class Mu chimeric GST Isoenzymes M1-2 And M2-1	84	25858	14	2	0.00017 to 0.011
	3	77628000	Nitrilase family, member 2	57	31024	3	1	0.0021
1b	1	77628000	Nitrilase family, member 2	323	31024	25	6	3.7 × 10−9 to 0.0002
2b	1	33356830	Crystal structures of class Mu chimeric GST Isoenzymes M1-2 And M2-1	246	25858	26	4	1.5 × 10−12 to 0.024
	2	204499	Glutathione S-transferase Y-b subunit (EC 2.5.1.18)	210	22042	36	5	4.7 × 10−6 to 0.024
	3	204501	Glutathione S-transferase (EC 2.5.1.18)	162	25840	11	2	1.5 × 10−12 to 0.0043
	4	77628000	Nitrilase family, member 2	67	31024	3	1	0.00027
3	1	77628000	Nitrilase family, member 2	161	31024	15	3	2 × 10−7 to 0.0096
	2	136429	Trypsin precursor	61	25078	8	1	0.0093
	3	204499	Glutathione S-transferase Y-b subunit (EC 2.5.1.18)	58	22042	7	1	0.0029
	4	220684	Cytosolic aspartate aminotransferase	46	46627	2	1	0.028

Blank piece of SDS gel – no significant hits

Blank piece of Native gel – no significant hits