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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Biochimie. 2009 Jul 10;91(9):1072–1080. doi: 10.1016/j.biochi.2009.07.003

Table 2.

Specific Activity of ω-amidase in mouse liver cytosol

Substrate Fhit−/− (FKO) Fhit−/−/Nit−/− (DKO) Control
Experiment 1 αKGM 15.9±0.7 14.7±0.7 17.5±0.5
Experiment 2 αKGM 18.5±1.3 18.7±1.5 18.5±1.1
Experiment 3 Succinamate 33.5±0.5 30.9±0.9 30.9±1.4

Specific activity is expressed as nmol/min/mg of protein; 37°C. Each value is the average ± s.d.; N = 3. Conversion of αKGM to α-ketoglutarate was measured in experiments 1 and 2. Hydroxaminolysis of succinamate was measured in experiment 3. Experiment 1. The reaction mixture contained liver extract (25 μg protein) incubated for 45 min. Experiment 2. The reaction mixture contained liver extract (100 μg of protein) incubated for 10 min. Experiment 3. The reaction mixture contained liver extract (100 μg of protein) incubated for 2 h.