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. Author manuscript; available in PMC: 2010 May 27.
Published in final edited form as: Transplantation. 2009 May 27;87(10):1488–1496. doi: 10.1097/TP.0b013e3181a43f18

Figure 1.

Figure 1

LLC-PK Cells transfected with the plasmid pEX Ezrin-YFP express YFP and ezrin. The transfected cell line LLC-EZ expressing YFP under fluorescence microscopy (A) and sham transfected LLC-PK1 cells showing only background autofluorescence (B). A representative Western blot shows the expression of Ezrin protein after cold storage and warm reperfusion (C). There is strong expression of Ezrin protein in fresh LLC-EZ cells. The expression of ezrin decreased to 15.3% of the baseline after 24 hours of cold preservation and recovered to 27.2% of the baseline after 1 hour of warm reperfusion. Comparatively little expression of Ezrin protein was observed in any of the LLC-PK cells.