Table 1.
Substrate Name | Amino acid sequence | ||||
---|---|---|---|---|---|
D motif | Linker | Consensus motif | Linker | DEF motif | |
ERKSub | aTGPLSPGPFc | ||||
MEK1ERK | aMPKKKPTPIQLNP | (AOO)3 | TGPLSPGPFc | ||
ERKFXFP | aTGPLSPGPF | (AOO)2 | FQFPc | ||
MEK1ERKFXFP | aMPKKKPTPIQLNP | (AOO)3 | TGPLSPGPF | (AOO)2 | FQFPc |
The N-terminus was covalently attached to fluorescein
(AOO) is the linker, 8-amino-3,6-dioxaoctanoyl
The C-terminus was amidated.