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. Author manuscript; available in PMC: 2010 Sep 18.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jul 15;387(2):414–418. doi: 10.1016/j.bbrc.2009.07.049

Table 1.

Designed peptide substrates for ERK

Substrate Name Amino acid sequence
D motif Linker Consensus motif Linker DEF motif
ERKSub aTGPLSPGPFc
MEK1ERK aMPKKKPTPIQLNP (AOO)3 TGPLSPGPFc
ERKFXFP aTGPLSPGPF (AOO)2 FQFPc
MEK1ERKFXFP aMPKKKPTPIQLNP (AOO)3 TGPLSPGPF (AOO)2 FQFPc
a

The N-terminus was covalently attached to fluorescein

b

(AOO) is the linker, 8-amino-3,6-dioxaoctanoyl

c

The C-terminus was amidated.