Figure 3. 3M-002 and resiquimod highly induce the secretion of Th1 associated cytokines and chemokines by LC previously incubated with or without HPV16 VLP.
Supernatants collected from untreated LC, LC exposed to HPV16 VLP, LC treated with each of the imidaziquinolines, or LC exposed to HPV16 VLP and then treated with imidaziquinolines were analyzed in triplicate for the presence of cytokines and chemokines. Cytokine and chemokine levels were quantified using a human cytokine LINCOplex assay. These data are expressed as the mean concentration with error bars representing the SD (*P < .05, ***P< .001). The experiment was repeated three times and yielded similar results.