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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: Cancer Res. 2009 Aug 4;69(16):6624–6632. doi: 10.1158/0008-5472.CAN-08-4101

Figure 3.

Figure 3

Induction of hCLCA2 by adenoviral transduction of p53. A, RT-qPCR shows 200-fold induction of hCLCA2 by 24h post-infection. Subconfluent MCF10A cells were infected with adenoviral supernatants at an m.o.i. of 30, and RNA was extracted and analysed. B, induction of hCLCA2 protein in parallel with p53. CA1d cells were infected with adenoviral supernatants and lysed at indicated intervals. Lysates were immunoblotted directly for p53 and tubulin or subjected to immunoprecipitation for hCLCA2 followed by immunoblotting. C, timing of hCLCA2 induction relative to p21. BT549 cells were infected and immunoblotted 20 or 40h later. Ad-GFP indicates Ad-Easy vector sans insert; p53, p53-Ad-Easy; h2, hCLCA2-Ad-Easy.