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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Cell Biochem. 2009 Jul 1;107(4):797–802. doi: 10.1002/jcb.22177

Fig. 5.

Fig. 5

Muc4/SMC is ubiquitinated in A375 cells. Protein from RIPA extracts of A375 or 13762 MAT-B1 ascites cells treated with or without proteosome inhibitors was subjected to sequential immunoprecipitation and immunoblot analyses with anti-ubiquitin and anti-Muc4. (A) Lysates immunoprecipitated with anti-ASGP-2 polyclonal antibody coupled to A/G-agarose and immunoprecipitates immunoblotted with anti-ubiquitin antibody. (B) Lysates immunoprecipitated with anti-ubiquitin antibody coupled to A/G-agarose and immunoprecipitates immunoblotted with 4F12 mAb.