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. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jul 15;387(3):516–520. doi: 10.1016/j.bbrc.2009.07.048

Figure 2. Glial activation decreased apoE production and secretion.

Figure 2

Primary mixed glial cultures were treated with LPS for 24 hrs. A) APOE mRNA was measured by Real-Time PCR. Relative Quantity of APOE expression shows that LPS treatment reduced APOE mRNA (mean ± SEM; *** P < 0.001; n = 6). B) Cell lysates and conditioned media were analyzed by Western blotting with antibodies to apoE and β-actin. Representative blots show that LPS reduced apoE levels in the cell lysate and conditioned media. C) Western blot data were quantified as percent of control apoE in the cell lysate or media. Cells treated with LPS showed a decrease in lysate apoE and secreted apoE compared with control untreated cells (mean ± SEM; * P < 0.05, *** P < 0.001 compared to corresponding control cultures; n = 6).