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. Author manuscript; available in PMC: 2010 Sep 25.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jul 15;387(3):516–520. doi: 10.1016/j.bbrc.2009.07.048

Figure 4. Inhibition of JNK in vivo increased apoE.

Figure 4

A) 5μl vehicle control (C), 5μl SP600125 (SP) (10mM), or PD98059 (PD) (10mM) was injected into the hippocampus of adult mice. Western blot analysis revealed that injection of SP into the hippocampus decreased phospho-c-Jun and phospho-JNK expression. ApoE levels in the ipsilateral hippocampus increased with SP injection, but remained unchanged with PD injection. Across samples β-actin levels remained unchanged. B) Quantification of Western blot analysis revealed the apoE levels increased by 49 % with SP injection compared to control injection (mean ± SEM, n = 3). C) 5 μl or 10 μl SP (10mM) or vehicle control (5 or 10 μl) was injected into the hippocampus of adult mice. Representative blots show that apoE and ABCA1 levels were similar in the contralateral hippocampus from animals injected with either vehicle (C), 5μl or 10μl SP. ApoE and ABCA1 levels in the ipsilateral hippocampus increased in animals injected with SP. Levels of β-actin remained unchanged. D) Quantification of Western blot analysis revealed a significant increase in apoE with SP600125 (Mean ± SEM; * P < 0.05, *** P < 0.001 compared to vehicle control injected animals; n = 4 for vehicle control, n = 4 for 5μl SP600125, n = 2 for 10μl SP600125 injection).