FIG. 2.

Cyp46 antibody recognizes Cyp46 by immunohistochemistry. COS7 cells were transfected with either control vector (A) or human Cyp46 (B) for 48 h. Immunostaining was performed using anti-Cyp46 antibody and Alexa 488 secondary antibody (green). Cell nuclei were counterstained with Topro-3 (blue), 20×. Scale bar = 100 μm. (C) In rat DIV16 hippocampal neurons, Cyp46 levels (green) were most pronounced in large, well-connected neurons with very high expression in the cytoplasm. Neuronal cells were identified using anti-MAP-2 antibody and Alexa 546 secondary (red). Some MAP-2-negative cells show low levels of Cyp46 expression (arrow), 63×. Scale bar = 20 μm.