FIG. 8.

Cyp46 levels increase in activated microglia. Primary rat microglia were either left untreated (A) or treated with 50 ng/mL LPS for 24 h (B). LPS resulted in a strong increase in Cyp46 expression (Alexa 488, green), as well as a proliferation of microglial cells. Cell nuclei were counterstained with Topro-3 (blue), 20×. Scale bar = 100 μm. (C) Relative levels of Cyp46 at the cell body showed a 28% increase according to Student's t-test (*Student's t-test, p < 0.0001; control, n = 197; LPS treatment, n = 269). Error bars (standard error of the mean).