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. Author manuscript; available in PMC: 2010 Jun 19.
Published in final edited form as: Vaccine. 2009 May 3;27(30):3992–4000. doi: 10.1016/j.vaccine.2009.04.035

Figure 5. One-step growth curve.

Figure 5

A549 cells were infected with recombinant CVB3 virus at an MOI of 3 using titered stocks produced by transfection. Squares (■) represent CVB-MA, open squares (□) represent CVB-MA M1–10, triangles (▲) represent CVB3.1, circles (●) represent CVB-CA, and open circles (○) represent CVB-CA M1–10. CVB-MA and the M1–10 version, in which the HIV-1 matrix protein is stable in both for at least 10 passages, had similar growth dynamics to the parental virus (CVB3.1). Titers of the three viruses peaked at 8 h post-infection. The CVB-CA vectors had slower growth rates than the parental virus, taking 12–24 hours to reach peak titer, but eventually achieve a similar titer by end of assay.