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. Author manuscript; available in PMC: 2010 Jun 16.
Published in final edited form as: Biochemistry. 2009 Jun 16;48(23):5291–5302. doi: 10.1021/bi802321m

FIGURE 5.

FIGURE 5

Time dependent thermal inactivation plots (Et/E0 vs time) of WT and the mutant enzymes at 60 °C. It is assumed that Et/E0 goes to zero in calculating the rate constants for inactivation.