. Author manuscript; available in PMC: 2010 Jun 16.

Published in final edited form as: Biochemistry. 2009 Jun 16;48(23):5291–5302. doi: 10.1021/bi802321m

Table 1.

Kinetic parameters of human ASL enzymes at 25 °C

Enzyme	V_max ± S.E^a (μmol/min/mg)	K_0.5 ± S.E^a (μM)	Hill coefficient^a
WT	11.9 ± 0.1	2.02 ± 0.04	1.9 ± 0.3
R194C	12.0 ± 0.9	2.2 ± 0.3	1.3 ± 0.2
K246E	0.23 ± 0.03	0.8 ± 0.2	0.5 ± 0.1
L311V	8.6 ± 0.7	3.4 ± 0.5	1.3 ± 0.1
R396H	1.9 ± 0.2	3.8 ± 0.8	0.9 ± 0.2
R396C	2.0 ± 0.2	1.1 ± 0.2	0.9 ± 0.1

The V_max, K_0.5, and Hill coefficient values were determined by varying the concentration of SAMP and fitting the data to the Hill equation using Sigma Plot. The values are shown along with their standard errors.