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. Author manuscript; available in PMC: 2010 Aug 15.
Published in final edited form as: J Cell Biochem. 2009 Aug 15;107(6):1139–1149. doi: 10.1002/jcb.22216

Figure 5.

Figure 5

KiSS1 inhibited RhoA activation (GTP-bound RhoA) and RhoA-mediated cell migration. (A) Cells treated with KP10 and/or TNFα for 24hr and then subjected to the RhoA activity assays. TNFα-induced RhoA activity (GTP-bound RhoA) was inhibited by different concentrations of KP10. (B) Cells were transfected with empty vector, RhoA G14V, or RhoA T19N for 24hr, transferred to the upper chamber precoated with gelatin, and then stimulated with control peptide or KP10 for another 24hr. TNFα was added in the bottom chamber for 24hr in order for the chemotatic attractance. Mean and standard deviations represent data from triplicate experiments (*, p<0.05).