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. Author manuscript; available in PMC: 2010 Sep 4.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jun 25;386(4):715–717. doi: 10.1016/j.bbrc.2009.06.111

Fig. 1. The gene trap mutation in the mouse RFX1 gene.

Fig. 1

The pGT2Lxf vector was integrated into the second intron of the RFX1 gene that consists of 21exons. The locations of RFX1-specific and vector-specific primers used in PCR-based genotyping of embryos and mice are shown in the top panel. SA: splice acceptor sequence; PA: polyadenylation signal. A representative gel image of the PCR products is shown in the bottom panel. The wild-type and mutant alleles produce products of 620 and 385 nucleotides, respectively.