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. Author manuscript; available in PMC: 2009 Sep 16.
Published in final edited form as: Aging Cell. 2007 Feb;6(1):111–119. doi: 10.1111/j.1474-9726.2006.00266.x

Fig. 4.

Fig. 4

Effect of inhibition of ifg-1 and rsks-1 on resistance to various stresses. (a) One-day-old adult N2 and rsks-1(ok1255) worms were exposed to control RNAi, ifg-1(RNAi), and daf-2(RNAi) for 48 h in 20 °C before heat stressed at 35 °C. N2 on control RNAi (n = 43) N2 ifg-1(RNAi) (n = 44); N2 on daf-2(RNAi) (n = 21) rsks-1(ok1255) on control RNAi (n = 43), rsks-1(ok1255) ifg-1(RNAi) (n = 44), rsks-1(ok1255) daf-2(RNAi) (n = 30). Similar results were obtained in three experiments. (b) Adult worms were exposed to control RNAi, rsks-1(RNAi), and ifg-1(RNAi) for 48 h in 20 °C before being transferred to NGM agar plates containing 240 μm juglone. Mean survival was 3.5 h (n = 81) for N2 on control RNAi; 1.7 h (n = 66) for rsks-1(RNAi) (P < 0.0001); and 3.6 h (n = 74) for ifg-1(RNAi). (c) Worms treated with RNAi bacteria for 48 from the L4 stage were exposed to 2000 J m-2 of UV via a Stratalinker® 1800. Mean survival was 3.7 days (n = 47) for N2 on control RNAi; 3.4 days (n = 43) for rsks-1(RNAi); and 4.5 days (n = 57) for ifg-1(RNAi) (P < 0.0005). (d) N2 and rsks-1(ok1255) were both exposed to control RNAi, rsks-1(RNAi), and ifg-1(RNAi) for 48 h before being starved in S-basal. Standard deviations were calculated from five biological replicates. Between 50 and 100 worms per replicate were scored each day. Median survival was 6.4 days for N2 on control RNAi; 8.2 days for rsks-1(RNAi); and 10 days for ifg-1(RNAi). Similar results were obtained in at least three experiments.