Figure 2.

Enzymatic activities in transfected HEK293T cells. A, Acid β-mannosidase, acid β-galactosidase and E. coli β-galactosidase activities were measured in lysates of untransfected HEK293T cells and cells over-expressing either the wild-type (pCS2-MANBA) or the mutant (pCS2-MANBA-p.Arg641His) acid β-mannosidase. Activities are expressed as nmol/h.mg protein, and are means ± SEM of 3 independent experiments performed in duplicate. The β-mannosidase activity was similar in untransfected cells and cells transfected only with the vector encoding the bacterial β-galactosidase (data not shown).