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Expression of endogenous and overexpressed β-mannosidase. Lysates of control and patient's lymphoid cells (100 μg protein), as well as HEK293T cells transiently transfected with a vector encoding wild-type (pCS2-MANBA) or mutant (pCS2-MANBA-p.Arg641His) β-mannosidase (25 μg protein), were assessed for β-mannosidase expression by Western-blot analysis. NT, not transfected.
