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. 2009 Aug 27;2:28. doi: 10.1186/1756-6606-2-28

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Copyright © 2009 Guilding et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Micro-dissected Arc complex and DMH maintain circadian rhythms in PER2::LUC expression in normal and TTX containing media. EM-CCD images of micro-dissected Arc complex (Arc/ME/PT) (A) and DMH (B) captured before total bioluminescence was recorded in photomultiplier tubes (PMTs). (C; Arc complex) and (D; DMH) are detrended PMT recordings of relative PER2::LUC bioluminescence (counts per minute) emitted from the slices shown in (A) and (B). Inhibition of sodium channel dependent action potentials with TTX (0.5 μM) in micro-dissected Arc complex (F) and DMH (E) does not inhibit circadian rhythms in PER2::LUC expression.