Table 1.
Transcript | Forward | Reverse | Size |
RCAN1 All | 5'GGACATCACCTTTCAGTATT3' | 5'TTCCTCTTCTTCCTCCTTCT3' | 394 bp |
RCAN1-1 | 5'ACCATCGCCTGTCACCTGGA3' | 5'GGTGATGTCCTTGTCATACGTCCT3' | 96 bp |
RCAN1-4 | 5'CTCCCTGATTGCCTGTGTGG3' | 5'TTCCTCTTCTTCCTCCTTCT3' | 484 bp |
β actin | 5'TCATGAAGTGTGACGTTGACATCCGT3' | 5'CTTAGAAGCATTTGCGGTGCACGATG3' | 285 bp |
RCAN1 All = total RCAN1. Forward primers for RCAN1 All, RCAN1-1 and RCAN1-4 correspond to exons 5, 1 and 4 respectively. A common reverse primer for RCAN1 All and RCAN1-4 corresponds to exon 7, while the reverse primer for RCAN1-1 corresponds to exon 5.