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. 2009 Sep 2;4:6. doi: 10.1186/1750-2187-4-6

Table 1.

PCR Primers

Transcript Forward Reverse Size
RCAN1 All 5'GGACATCACCTTTCAGTATT3' 5'TTCCTCTTCTTCCTCCTTCT3' 394 bp
RCAN1-1 5'ACCATCGCCTGTCACCTGGA3' 5'GGTGATGTCCTTGTCATACGTCCT3' 96 bp
RCAN1-4 5'CTCCCTGATTGCCTGTGTGG3' 5'TTCCTCTTCTTCCTCCTTCT3' 484 bp
β actin 5'TCATGAAGTGTGACGTTGACATCCGT3' 5'CTTAGAAGCATTTGCGGTGCACGATG3' 285 bp

RCAN1 All = total RCAN1. Forward primers for RCAN1 All, RCAN1-1 and RCAN1-4 correspond to exons 5, 1 and 4 respectively. A common reverse primer for RCAN1 All and RCAN1-4 corresponds to exon 7, while the reverse primer for RCAN1-1 corresponds to exon 5.