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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: Anal Biochem. 2009 Jul 16;394(2):164–170. doi: 10.1016/j.ab.2009.07.021

Figure 2. Enhanced detection limits of LC-MS.

Figure 2

Multiple reaction monitoring (MRM) increases sensitivity by detecting only the fragments derived from a specified parent ion. The MS2 chromatogram depicted here shows the signal intensity of a fragment (m/z 534.3) derived from the main parent ion of vasopressin (ADH: m/z 543.0). The signal intensity from a predominant fragment may be extracted and used for quantification. The log × log plot insert of regression analysis shows linearity of response over 4 orders of magnitude down to 10−17 mol injected on column.