Figure 3.

zwf mutation suppresses both msbB-induced CO₂ sensitivity and osmotic defects. Double velvet replica plates with different media were used to indicate the ability of small patches of bacteria (3 each) to grow. The strains used are listed on the left. Growth conditions (all at 37°C) included: A, LB media in air; B, LB media in 5% CO₂; C, MSB media in air; D, MSB media in 5% CO₂; E, LB-0 media in air; F, LB-O media in 5% CO₂; G, LB-0 media containing sucrose (total 455 miliosmoles) in air; H, LB-0 media containing sucrose in 5% CO₂; I, LB-0 + gluconate (glucon.) in air; J, LB-0 + gluconate in 5% CO₂.