Table 2.
Distribution of protein expression of the apoptotic and antiapoptotic markers, Cox proportional hazard ratios (HR) and 95% confidence intervals (CI) for overall survival by the biomarker status for ESCC patients.
| Gene protein | N1 | Distribution of protein expression by overall score (N, prevalence)2 | HR3 (95% CI) P-value |
Number (prevalence) with positive protein expression score4 | HR5 (95% CI) P-value |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Score | |||||||||||||
| 0 | 1 | 2 | 3 | 4 | 6 | 8 | 9 | 12 | |||||
| FasL | 244 | 15 (0.06) |
48 (0.20) |
92 (0.38) |
80 (0.33) |
3 (0.01) |
6 (0.03) |
--- | --- | --- | 1.10 (0.95-1.27) 0.45 |
9 (0.04) | 1.04 (0.45-2.40) 0.92 |
| Fas | 244 | 24 (0.10) |
49 (0.20) |
44 (0.18) |
91 (0.37) |
8 (0.03) |
22 (0.09) |
--- | 5 (0.02) |
1 (0.00) |
0.95 (0.87-1.04) 0.07 |
36 (0.15) | 0.79 (0.49-1.27) 0.32 |
| FADD | 248 | 10 (0.04) |
122 (0.49) |
72 (0.29) |
8 (0.03) |
17 (0.07) |
12 (0.05) |
4 (0.02) |
1 (0.00) |
2 (0.01) |
1.05 (0.96-1.14) 0.79 |
36 (0.15) | 1.22 (0.77-1.92) 0.39 |
| pFADD | 247 | 190 (0.77) |
48 (0.19) |
9 (0.04) |
--- | --- | --- | --- | --- | --- | 1.02 (0.76-1.38) 0.25 |
0 (0.00) | --- |
| Caspase 8 | 244 | 11 (0.05) |
76 (0.31) |
144 (0.59) |
--- | 11 (0.05) |
2 (0.01) |
--- | --- | --- | 0.92 (0.76-1.11) 0.53 |
13 (0.05) | 1.03 (0.51-2.05) 0.94 |
| Caspase 10 | 235 | 6 (0.03) |
47 (0.20) |
161 (0.69) |
--- | 17 (0.07) |
3 (0.01) |
--- | 1 (0.00) |
--- | 1.07 (0.93-1.23) 0.46 |
21 (0.09) | 1.36 (0.81-2.27) 0.25 |
| Bcl2 | 253 | 23 (0.09) |
167 (0.66) |
58 (0.23) |
--- | 5 (0.02) |
--- | --- | --- | --- | 1.01 (0.81-1.27) 0.63 |
5 (0.02) | 1.29 (0.52-3.25) 0.58 |
1N shown is for each individual biomarker and is less than 265 total because none of 7 biomarkers had data for all 265 ESCC cases.
2Overall score calculated as (intensity score) times (percent cells positive score) as described in methods.
3HR = hazard ratio (95% confidence interval) for increase in score of one category; from multivariate Cox proportional hazards model, adjusted for gender, age, tobacco use, alcohol use, family history of UGI cancer, tumor grade, tumor stage, metastasis, and degree differentiation.
4Positive protein expression = overall score ≥ 4 (negative = overall score ≤ 3).
5HR = hazard ratio (95% confidence interval) for positive protein expression (versus negative); from multivariate Cox proportional hazards model, adjusted for gender, age, tobacco use, alcohol use, family history of UGI cancer, tumor grade, tumor stage, metastasis, and degree differentiation.