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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: Dev Biol. 2009 May 3;331(2):167–175. doi: 10.1016/j.ydbio.2009.04.032

Figure 5. BMPRII deletion in endocardial lineage cells using a Tie2-Cre transgene.

Figure 5

(A, B) Staining for β-galactosidase activity (blue) with nuclear fast red counterstaining (pink) was performed at P2. Tie2-Cre activity was detected in the membranous portion of the interventricular septum in control mice (A, arrow). In BMPRIIflox/−;Tie2-Cre mutant mice at P2, the membranous portion of the septum failed to connect to the muscular portion of the septum (B, arrow). Mitral valve leaflets stained positively for β-galactosidase activity (B). Hematoxylin and eosin staining of a mitral valve from a control mouse (C) and a BMPRIIflox/−;Tie2-Cre mutant mouse (D) at P2 is shown. RV: right ventricle, LV: left ventricle, IVS: interventricular septum, Ao: aorta, LA, left atrium, MV: mitral valve. Scale bars: 0.2 mm (A, B) and 0.1 mm (C, D).