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. Author manuscript; available in PMC: 2010 Sep 29.
Published in final edited form as: J Neuroimmunol. 2009 Jul 7;214(1-2):25–32. doi: 10.1016/j.jneuroim.2009.06.010

Figure 2. Stimulation of microglia with LPS increased NO through JNK activation.

Figure 2

A) In wild-type and LRP1 −/− microglia, LPS promoted nitrite accumulation in the conditioned media at 24 h (mean ± SEM; ***P < 0.001; n = 6 for each of 3 independent experiments). B) Cell lysates from wild-type and LRP1 −/− microglia were analyzed by Western blotting with antibodies to iNOS and β-actin (as a control). A representative blot shows that LPS increased iNOS levels in both cell types (n=6 for each of 3 independent experiments). C) Wild-type and LRP1 −/− microglia were treated with LPS alone (open bars) or LPS and SP600125 (colored bars). In a dose-dependent manner, SP600125 treatment reduced nitrite production in both wild-type and LRP1 −/− microglia to levels of unstimulated microglia (defined as the line at 100 %) (mean ± SEM; **P < 0.01 compared to control cultures; # P < 0.05, ## P < 0.01 compared to corresponding cultures; n=3 for each of 2 independent experiments).