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. Author manuscript; available in PMC: 2010 Sep 4.
Published in final edited form as: Vaccine. 2009 Jul 17;27(40):5488–5495. doi: 10.1016/j.vaccine.2009.06.103

Figure 2. Gating strategy used to analyze CD4 and CD8 T cell cytokine responses.

Figure 2

Whole blood was incubated with BCG to complete a flow cytometric intracellular cytokine assay, as described in the Methods.

(A) Doublet cells were excluded by gating on Forward Scatter–Area (FSC-A) against Forward Scatter-Height (FSC-H), and lymphocytes were then selected in a FSC-A against Side Scatter-Area (SSC-A) dots plot. T cells were selected by gating on CD3+ events, which were further differentiated into CD4 and CD8 T cells. (B) Representative dot plots of cytokine co-expression patterns in CD4 T cells from unstimulated, BCG and SEB-stimulated conditions.