Skip to main content
. 2009 Oct 2;5(10):e1000603. doi: 10.1371/journal.ppat.1000603

Figure 3. Cytochalasin D treatment protects gastric epithelial cells against both drop in mitochondrial transmembrane potential and apoptosis induced by VacA by preventing its mitochondrial localization.

Figure 3

(A) Effect of cytochalasin D (CD) on mitochondrial transmembrane potential (MTP) of MKN 28 cells, treated or not with VacA, evaluated by using the ratiometric potential-sensitive dye JC-1 and confocal microscopy. All the pictures shown represent single confocal sections. Scale bar: 10 µm. The graph (right) shows the quantitation (mean±SEM) of relative MTP in the different experimental conditions by extensive confocal microscopy evaluation of slides from 3 independent experiments. *: P<0.05 versus paired control. °: P<0.05 versus the same condition in the presence of CD. (B) Morphology of mitochondria and their colocalization with VacA in AGS cells transfected with DsRed-Mito and treated or not with Cy5-labelled VacA in the absence or presence of CD. DsRed-Mito (red) and Cy5-VacA (green). All the pictures shown represent single confocal sections. Scale bar: 10 µm. (C) Apoptosis (shown as fold increase over the CD-untreated control cells) induced by VacA in MKN 28 cells treated or not with CD. Cells treated or not with CD but not exposed to VacA served as controls. Mean±SEM of three different experiments. *: P<0.05 versus paired control. °: P<0.05 versus the same condition in the presence of CD.