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. 2009 Oct;11(10):1003–1011. doi: 10.1593/neo.09688

Figure 1.

Figure 1

Acetaminophen reduced cellular GSH levels in HUH6 cells in a dose-response manner. (A) HUH6 cells were treated with 5, 10, or 20 mM AAP, with or without 1 mg/ml of NAC, and collected the following day (n = 3, mean ± SEM). GSH levels were analyzed using a Quanticrom Glutathione Assay Kit and normalized for protein concentration. (B) HUH6 cells were treated with AAP and then were stained using a ThiolTracker Violet dye from Invitrogen. Shown are representative pictures of cells from each treatment group. Statistical significance between treatment and control group was indicated by asterisks: *P < .05 or **P < .01.