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. 2009 Oct;11(10):1054–1063. doi: 10.1593/neo.09762

Figure 3.

Figure 3

Analysis of endothelial adhesion receptor expression. HUVECs were cocultivated with UKF-NB-4. CD56+ populations were gated out by flow cytometry (R1 vs R2) as described in the Materials and Methods section (A). E-selectin (CD62E), ICAM-1 (CD54), and P-selectin (CD106; B), and CD44v4, v5, and v7 (C) of CD56-negative HUVECs were then evaluated by FACS analysis (MFU indicates mean fluorescence units). “HUVEC” depicts isolated, nonconditioned endothelial cells (controls), MFU of which were set to 100%. “HUVEC/NB” indicates endothelial cells preconditioned by UKF-NB-4 tumor cells. One representative of n = 6 experiments. *Significantly different from control HUVECs.