Figure 5.

(A) Western blot analysis using an anti-HIF-1α mAb of protein extracts from A375 hypoxic cells (1% O₂) under VP-16 10 µM and doxorubicin (Doxo) 1 µM treatment (4 hours) in the absence and in the presence of the A₃ adenosine receptor antagonist MRE 3008F20 1 µM. HIF-1β shows equal protein loading. (B) The mean densitometric data from three independent experiments were normalized to the results obtained in cells in the absence of drugs (control). Plots are mean ± SE values (n = 3). *P < .01 compared with control without chemotherapeutic treatment; analysis was by ANOVA followed by Dunnett test. ^#P < .01 compared with the cells exposed to chemotherapeutic alone (2 vs 5 and 3 vs 6 for VP-16 and doxorubicin, respectively). Analysis was by t test.