Figure 2. A–D. Inhibition of NOS1, NOS2, and NOS3 enzymatic by cobinamides and cobalamins.
The enzymatic activity of purified NOS1, NOS2, or NOS3 was assessed in presence or absence of different concentrations of (A) hydroxocobalamin, (B) cobinamide, (C) dicyanocobinamide, or (D) cyanocobalamin. Results are expressed as percent inhibition. The estimated effective dose for 50% inhibition (ED50) is displayed. Note the differing scales of the x axes.
