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. Author manuscript; available in PMC: 2010 Jun 15.
Published in final edited form as: Free Radic Biol Med. 2009 Mar 27;46(12):1626–1632. doi: 10.1016/j.freeradbiomed.2009.03.017

Figure 4. Effect of dicyanocobinamide on the UV-visible spectra of purified, recombinant NOS1 oxygenase domain.

Figure 4

Binding of 40 μM CN2Cbi to NOS1 oxygenase domain (NOS1ox; ~ 10 μM) in 40 mM bis- tris-propane buffer (pH 7.5) containing 5% glycerol and 10 μM H4B with at room temperature. The solid line represents NOS1ox alone, and the dashed line NOS1ox with CN2Cbi after 200 minutes’ interaction. The inset displays the difference spectrum generated by subtracting NOS1ox initial spectrum from the spectrum after 200 minutes with CN2Cbi.