Table 1. Inhibition of nitric oxide synthases by cobalamins and cobinamides.
The effects of the indicated agents on the enzymatic activity of NOS1 and NOS2 were determined. Methyl-cobalamin and adenosylcobalamin were tested either having been exposed to light or not exposed to light. Results are displayed as the ED50 (μM).
| Estimated ED50 (μM) | |||
|---|---|---|---|
| NOS1 | NOS2 | NOS3 | |
| Hydroxocobalamin | 5.7 | 21.1 | 9.1 |
| Cobinamide | 15.0 | 50.0 | 43.0 |
| Dicyanocobinamide | 0.23 | 2.29 | 0.33 |
| Cyanocobalamin | 72.0 | 1620.0 | 519.0 |
| Glutathionylcobalamin | 9.6 | 29.0 | 41.0 |
| Carboxy-PTIO | 1360.0 | 1359.0 | - |
| Methylcobalamin (no light) | 576.0 | 231.0 | - |
| Methylcobalamin (with light) | 5.9 | 63.0 | - |
| Adenosylcobalamin (no light) | 498.0 | 2148.0 | - |
| Adenosylcobalamin (with light) | 6.4 | 60.0 | - |