. Author manuscript; available in PMC: 2010 Sep 1.

Published in final edited form as: Biochemistry. 2009 Sep 1;48(34):8143–8150. doi: 10.1021/bi900773r

Table 1.

Apparent thermodynamic parameters for dissociation of subunits from CcO derived from urea, or GdmCl-induced, isothermal dissociation data acquired at room temperature

Subunit	10 min Exposure to Urea		120 min Exposure to Urea		10 min GdmCl Exposure
	ΔG^H2O (kJ/mol)	[urea]_1/2^a (M)	ΔG^H2O (kJ/mol)	[urea]_1/2^a (M)	ΔG^H2O (kJ/mol)	[GdmCl]_1/2^a (M)
VIb	18.8±1.1	3.7	14.4±1.4	3.5	18.3±3.1	1.03
VIa	18.0±2.6	3.8	14.2±1.4	3.6	19.3±3.2	1.04
VIIa	24.7±3.0	4.4	13.7±1.2	3.9	14.6±2.6	1.12
III	29.7±3.1	4.8	12.3±1.4	3.9	14.9±1.5	1.14
Vb	---	---	19.9±1.9	4.9	---	---

[urea]_1/2 = ΔG^H2O/m; standard error in m = 5-10%; standard error in [urea]_1/2 = 15-20%.

[GdmCl]_1/2 = ΔG^H2O/m; standard error in m = 5-10%; standard error in [GdmCl]_1/2 = 15-20%.