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. Author manuscript; available in PMC: 2009 Sep 17.
Published in final edited form as: Exp Biol Med (Maywood). 2008 Apr 29;233(7):849–859. doi: 10.3181/0712-RM-352

Figure 2.

Figure 2

Results of alanine scanning mutagenesis on the binding of the long cytoplasmic domain of CEACAM1 with β-catenin. The wild type sequence of the long cytoplasmic domain is shown on the x-axis and the results of a quantitative yeast two-hybrid assay (± SED, 4 replicates) shown on the y-axis. Red bars. minus bait; blue bars, plus bait; Δ, truncated version (451–484) of the long cytoplasmic domain; Φ, wild type version of CEACAM1 long cytoplasmic domain; Γ, 1-60hTcf-4 as the positive control. Except for mutants H469A, K470A, and D479A, P < 0.001 (bait vs. no bait); thus, identifying these 3 residues as hot spots for binding β-catenin.